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Stability Testing Standards

Posted on Nov 30, 2016 in Blog

stability chamberThe development of pharmaceuticals is a lengthy ordeal. Large amounts of time and money are devoted to the process of testing the efficacy of a new drug in patients. However, it is also important to test that the drug will remain effective after it has spent several months sitting on the shelf in a pharmacy. Standards and practices are necessary to make sure that medications are comprehensively tested for potency under long-term and potentially stressful environmental conditions.

The International Council for Harmonisation (ICH) is the international body that develops these standards. The ICH was formed in 1990 to fill the need to have a unified process for evaluation of new medical products between Europe, Japan, and the United States.  Their goal is to provide “recommendations towards achieving greater harmonisation in the interpretation and application of technical guidelines and requirements for pharmaceutical product registration, thereby reducing or obviating duplication of testing carried out during the research and development of new human medicines.”

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Searching for Answers to Alzheimer’s via the Microbiome

Posted on Oct 26, 2016 in Blog

amyloid beta proteinAs medical technologies continue to develop, the average human life span is increasing along with them. The world population is getting older on average, a trend that will continue as members of the Baby Boom generation enter their later years. However, as humans live longer and longer, they are more at risk for certain diseases that tend to manifest later in life. One of the diseases we are increasingly susceptible to is Alzheimer’s disease. Alzheimer’s disease is a chronic neurodegenerative disease affecting 48 million people as of 2015; about 6% of people over the age of 65 are affected. It is expected, but not certain, that genetics play a role in the risk of developing Alzheimer’s. The symptoms of the disease usually start out as relatively mild, often manifesting as problems with short-term memory. As the disease progresses, symptoms escalate to include disorientation (Alzheimer’s is responsible for up to 70% of the cases of dementia), loss of motivation, problems with language, poor self-care, mood swings, and behavioral issues. Alzheimer’s is one of the costliest diseases in our society, racking up more than $100 billion annually in charges for treatment such as nursing home care, in-home day care, and indirect costs of locating lost patients and caregiver productivity. There is currently no cure for Alzheimer’s, although treatments such as physical and mental exercise programs may temporarily improve symptoms.

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Zebrafish and the Microbiome

Posted on Sep 27, 2016 in Blog

lactobacillus caseiHuman beings can never truly be alone. Even when apart from other humans, we still share our body with trillions of microorganisms. In fact, there are likely more non-human cells in your body than there are human cells; the most recent estimates of that ratio approximate that you have three non-human cells in our body for every human cell. This complex system of microbial organisms living inside us is referred to as the microbiome. Some of these bacteria live in your mouth or on your skin, but a majority of them (around 100 trillion or so) live in the gastrointestinal tract. Most of these are beneficial, and include bacteria such as Bacteroides fragilis, Helicobacter pylori, Lactobacillus casei, and Lactobacillus reuteri. This gut microbiome can have far reaching impacts on the human body. Imbalances in the composition of the gut microbiome, or “flora,” have been shown to impact the immune system, metabolism, digestion, and even brain function. This means that, in addition to obvious things like intestinal diseases and infections (such as CDI, or Clostridium difficile infection, which can occur when overuse of antibiotics kills off so much beneficial gut bacteria that the toxic C. difficile can spread rampantly), imbalances in gut flora can also lead to things like depression.

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A Possibility of Prions in Plants

Posted on Aug 31, 2016 in Blog

prionIn the 1960’s, two researchers in London were investigating why diseases like scrapie and Creutzfeldt–Jakob disease (CJD) resisted ionizing radiation. What they hypothesized was that these diseases were caused by proteins, rather than a biological agent. However, it wasn’t until the 1980’s that these hypothetical proteins, dubbed prions, were isolated and purified. Prions are proteins that can fold in multiple structurally distinct ways. These folds can be transferred to other prion proteins, and this propagation results in diseases similar to bacterial infections. In addition to scrapie and CJD (a human disease that causes brain tissue to rapidly decay, leaving the brain with a sponge-like texture), prions are also suspected as the cause of bovine spongiform encephalopathy (BSE, a.k.a. “mad cow disease”).

Currently, all of the known prion diseases in mammals target either the brain or neural tissue. Since prion proteins are able to transfer their folded state to normal versions of the protein, treatment methods for prion disease would involve denaturing the proteins: un-twisting them back into their natural state so that they are no longer able to induce folding of other proteins. However, a practical method to do this doesn’t currently exist, so prion diseases are untreatable – and always fatal.

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The Science of C. elegans

Posted on Jul 25, 2016 in Blog

C elegansIn 1963, Dr. Sydney Brenner, a South African biologist, went looking for a model organism to advance the study of biological development, specifically targeting the nervous system. What he found was Caenorhabditis elegans, or C. elegans for short. C. elegans is a small, free-living (i.e. non-parasitic) roundworm. Dr. Brenner chose C. elegans to be the model organism since it is one of the simplest organisms with a nervous system. The nervous system of every C. elegans specimen contains exactly 302 neurons, and this consistency between individuals makes them perfect for study. Studies with C. elegans have also branched out to include other fields such as embryogenesis, sex determination, and larval development. Research using C. elegans has led to discoveries in areas such as programmed cell death (a.k.a. apoptosis, which is an important process in the study of diseases like Leukemia), RNA interference, nicotine addiction (worms respond to nicotine similarly to the way mammals do), ageing research, and space research including zero gravity effects on development, muscle atrophy, etc.

C. elegans has a lot of characteristics that make it desirable for research in general. The specimens are small; they measure about 1 millimeter in length. They eat bacteria (such as E. coli), so they can be easily grown on agar plates with up to 10,000 worms on a single petri dish. Most adult C. elegans specimens are hermaphroditic, and each hermaphroditic worm can produce 300-350 offspring, so breeding for specific mutations can be done relatively easily. They also have short development times (3 days from egg to adult) and life spans (2-3 weeks), so studying genetic effects across generations is possible over short time periods. C. elegans can also survive while frozen in liquid nitrogen, making long-term storage of specific mutations possible for future studies.

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Light Intensity in Rodent Incubators

Posted on Jun 23, 2016 in Blog

Many rodents are nocturnal. However, even rodents that are active during the day tend to prefer darker areas. Most rats, for example, have adapted to be accustomed to spending daylight hours largely sheltered from light sources. This makes lighting control in habitats used for rodent research an important variable to be considered. However, lighting control encompasses more than just light intensity. Things like the duration of exposure, pigmentation of the animal, age, species, and sex of the animal are just some of the other factors that need to be considered. Additionally, many rodents used in research applications are albino, which makes them more susceptible to light than other varieties. Because of this, albino rodents have been used in establishing baselines for illumination levels.

rodent incubator light coverSo what light levels are appropriate for housing rodents? According to the US National Research Council’s Guide for the Care and Use of Laboratory Animals, the light experience of each animal can affect its light sensitivity, but in normal cases light intensity at the cage level should be between 12 and 30 foot candles (130-325 lux). The normal fluorescent bulbs in our Rodent Incubators produce light intensity between 80-100 foot candles (860-1080 lux) at the cage, which is quite strong. A glass door in the incubator (with no timed light control) could allow the room lighting to provide the correct illumination, but we wanted to provide a better option for researchers looking for less intense, and timed, lighting within the chamber. Powers Scientific now offers dimmable light covers on our Rodent Incubators. These light covers are plastic bulb sleeves with graded black striping to block some of the light emitted. The striping changes in density around the cover so that as the covers are rotated around the bulb, more or less of the light is blocked depending on the direction you twist it. With the light covers mounted, light intensity inside the chamber is reduced to 10-40 foot candles (105-430 lux), depending on how the covers are oriented. With these light covers, our chambers are capable of providing low-intensity lighting conditions for many applications.

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